000007543 001__ 7543
000007543 005__ 20240605130821.0
000007543 02470 $$2doi$$a10.24868/BWTC6.2017.012
000007543 035__ $$a1456909
000007543 037__ $$aGENERAL
000007543 245__ $$aATP assay for rapid onboard testing to detect living microorganisms in Ballast Water
000007543 269__ $$a2017-01-13
000007543 336__ $$aConference Proceedings
000007543 520__ $$aRapid and simple analytical methods for viable microorganism detection in ballast water are required to evaluate the efficiency of ballast water treatment system. During the course of systematic investigation of the cytotoxicity and apoptosis assays, it was found that the adenosine triphosphate (ATP) and luminescence based cell viability assay, in other word, an ATP assay was the most sensitive and applicable to ballast water management (BWM). The assay was applied to cultured microalgae samples, and it could detect the existence of 5 viable cells in 100 μl. Comparably low luminescent values were detected in two cultured diatom species than in cultured dinoflagellates. This result might be caused by the small cell volume in diatom species. Following a regression model between ATP concentration and cell volume, an ATP guideline (876 – 109246 relative luminescence units: RLU) was developed for the evaluation of treated ballast water. ATP assay was also applied to the evaluation of ballast water treatment system (BWTS). The luminescence value which obtained from the ATP assay also showed a good correlation with the presence of living natural plankton cells with comparably low luminescence values than the cultured species. The low ATP concentration in natural plankton cells may reflect a decline in their biological activity because of extended exposure to dark conditions. ATP assay could be a suitable method for the monitoring of ballast water management compliance even though the results of this study need further validation.
000007543 542__ $$fCC-BY-NC-ND-4.0
000007543 6531_ $$aBallast water management system (BWMS)
000007543 6531_ $$aAdenosine triphosphate (ATP)
000007543 6531_ $$aRelative luminescence units (RLU)
000007543 6531_ $$aballast water management compliance
000007543 7001_ $$aYum, Seungshic$$uSouth Sea Environment Research Center, Korea Institute of Ocean Science and Technology
000007543 7001_ $$aHyun, Bong Gil$$uBallast Water Center, Korea Institute of Ocean Science and Technology
000007543 7001_ $$aRhie, Kitae$$uDepartment of Biology, Kyung Hee University
000007543 7001_ $$aShin, Kyoungsoon$$uBallast Water Center, Korea Institute of Ocean Science and Technology
000007543 773__ $$jBWTC 2017
000007543 773__ $$tConference Proceedings of BWTC
000007543 789__ $$whttps://zenodo.org/record/1456909$$2URL$$eIsIdenticalTo
000007543 85641 $$uhttps://www.imarest.org/bwt$$yConference website
000007543 8564_ $$92226220d-3b95-45e6-829b-ec56691a9503$$s355600$$uhttps://library.imarest.org/record/7543/files/10_24868BWTC6_2017_012.pdf