000007536 001__ 7536
000007536 005__ 20240605130821.0
000007536 02470 $$2doi$$a10.24868/BWTC6.2017.005
000007536 035__ $$a1454303
000007536 037__ $$aGENERAL
000007536 245__ $$aCMOS-based Image Cytometry for Detection of Phytoplankton in Ballast Water
000007536 269__ $$a2017-01-13
000007536 336__ $$aConference Proceedings
000007536 520__ $$aAn image cytometer (CYT) for the analysis of phytoplankton in fresh and marine water environments is introduced. A linear quantification of the number of cells over several orders of magnitude of concentrations was observed using cultures of <em>Tetraselmis</em> and <em>Nannochloropsis</em> measured by autofluorescence of the chlorophyll in a laboratory environment. The functionality of the system outside the laboratory was analysed by phytoplankton quantification of samples taken from marine water environment (Dutch Wadden Sea, The Netherlands) and fresh water environment (Lake Ijssel, The Netherlands). The CYT was also employed to study the effects of two ballast water treatment systems (BWTS), based on chlorine electrolysis and UV sterilisation by determining the vitality of the phytoplankton. In order to ensure the detection limit, a large volume (1l) of samples was collected and concentrated to 3 ml using Celltrap<sup>TM</sup> filters. The results were compared to benchmarked flow cytometer and PAM Fluorometry at Marine Eco-Analytics (MEA-NL). The image cytometer reached a 10 cells/ml limit of detection (LoD) with an accuracy between 0.7 and 0.5 log, and a correlation of 88.29% in quantification and 96.21% in vitality, when compared to benchmarked monitoring techniques.
000007536 542__ $$fCC-BY-NC-ND-4.0
000007536 6531_ $$aBallast Water
000007536 6531_ $$aimage Cytometer
000007536 6531_ $$aCytometry
000007536 6531_ $$aPhytoplankton
000007536 6531_ $$aCompliance Monitoring
000007536 6531_ $$aMaritime
000007536 7001_ $$aJofre, M$$uICFO – Institut de Ciencies Fotoniques, The Barcelona Institute of Sciences and Technology
000007536 7001_ $$aPérez, JM$$uLABAQUA
000007536 7001_ $$aMartínez, P$$uICFO – Institut de Ciencies Fotoniques, The Barcelona Institute of Sciences and Technology
000007536 7001_ $$aHurth, C$$uICFO – Institut de Ciencies Fotoniques, The Barcelona Institute of Sciences and Technology
000007536 7001_ $$aYánez, MA$$uLABAQUA
000007536 7001_ $$aCatalán, V$$uLABAQUA
000007536 7001_ $$aParker, A$$uMemTeq Ventures Ltd
000007536 7001_ $$aVeldhuis, M$$uMarine Eco Analytics
000007536 7001_ $$aPruneria, V$$uICREA – Institució Catalana de Recerca
000007536 773__ $$jBWTC 2017
000007536 773__ $$tConference Proceedings of BWTC
000007536 789__ $$whttps://zenodo.org/record/1454303$$2URL$$eIsIdenticalTo
000007536 85641 $$uhttps://www.imarest.org/bwt$$yConference website
000007536 8564_ $$9d01b7e09-cd19-4088-bed1-a6bda4c4de99$$s871090$$uhttps://library.imarest.org/record/7536/files/10_24868BWTC6_2017_005.pdf