@article{GENERAL,
      author = {Jofre, M and Pérez, JM and Martínez, P and Hurth, C and  Yánez, MA and Catalán, V and Parker, A and Veldhuis, M  and Pruneria, V},
      url = {http://library.imarest.org/record/7536},
      journal = {Conference Proceedings of BWTC},
      title = {CMOS-based Image Cytometry for Detection of Phytoplankton  in Ballast Water},
      abstract = {An image cytometer (CYT) for the analysis of phytoplankton  in fresh and marine water environments is introduced. A  linear quantification of the number of cells over several  orders of magnitude of concentrations was observed using  cultures of <em>Tetraselmis</em> and  <em>Nannochloropsis</em> measured by autofluorescence of  the chlorophyll in a laboratory environment. The  functionality of the system outside the laboratory was  analysed by phytoplankton quantification of samples taken  from marine water environment (Dutch Wadden Sea, The  Netherlands) and fresh water environment (Lake Ijssel, The  Netherlands). The CYT was also employed to study the  effects of two ballast water treatment systems (BWTS),  based on chlorine electrolysis and UV sterilisation by  determining the vitality of the phytoplankton. In order to  ensure the detection limit, a large volume (1l) of samples  was collected and concentrated to 3 ml using  Celltrap<sup>TM</sup> filters. The results were compared to  benchmarked flow cytometer and PAM Fluorometry at Marine  Eco-Analytics (MEA-NL). The image cytometer reached a 10  cells/ml limit of detection (LoD) with an accuracy between  0.7 and 0.5 log, and a correlation of 88.29% in  quantification and 96.21% in vitality, when compared to  benchmarked monitoring techniques.},
      number = {GENERAL},
      recid = {7536},
      address = {2017-01-13},
}